Unknown,Transcriptomics,Genomics,Proteomics

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Transcript Analysis of E. coli ATCC 35218 suggest a Role of cranberry Juice in Inhibiting the growth of E. coli

ABSTRACT: Several different mechanisms have been proposed to explain the possible role of cranberries, cranberry juice, and cranberry extracts in inhibiting bacterial growth. In this report, we showed that Escherichia coli showed slower growth rate in response to the presence of cranberry juice in the growth media. By compareing the global transcript profiles, significant modulation of several genes of E. coli grown in LB broth with 10% cranberry juice were identified and provided identification of the potential mechanisms involved in the inhibitory effects of cranberry juice. The results presented clearly demonstrate that the inhibitory effect on bacterial growth observed in the presence of cranberry juice/extracts is primarily a result of the iron chelation capacity of PACs and direct disruption of metabolic enzymes. The results are discussed with a focus on the genes associated with iron chelation capability. Keywords: growth inhibition of cranberry juice For transcriptome profiling, there were 15 Affymetrix GeneChipÂ® E. coli genome 2.0 arrays total. There were five conditions: E. coli grown in LB broth, E. coli grown in LB broth with 10% cranberry juice to generation 50, 160, 210, and 230. Each condition was done in triplicate. Five conditions done in triplicates resulted in 15 samples that went onto 15 microarrays.

ORGANISM(S): Escherichia coli

SUBMITTER: Robert Rubin

PROVIDER: E-GEOD-12554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Impact of cranberry on Escherichia coli cellular surface characteristics.

Johnson Brandy J BJ Lin Baochuan B Dinderman Michael A MA Rubin Robert A RA Malanoski Anthony P AP Ligler Frances S FS

Biochemical and biophysical research communications 20081026 3

The anti-adhesive effects of cranberry have been attributed to both interactions of its components with the surface of bacterial cells and to inhibition of p-fimbriae expression. Previous reports also suggested that the presence of cranberry juice changed the Gram stain characteristics of Escherichia coli. Here, we show that the morphology of E. coli is changed when grown in the presence of juice or extract from Vaccinium macrocarpon (cranberry). Gene expression analysis indicates the down regul ...[more]

PMID: 18957286

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Dataset Information

Transcript Analysis of E. coli ATCC 35218 suggest a Role of cranberry Juice in Inhibiting the growth of E. coli

Publications

Impact of cranberry on Escherichia coli cellular surface characteristics.

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