Transcription profiling of human dendritic cells exposed to microfilariae of Brugia Malayireveals induction of trail and TNF-alpha-dependent apoptosis

ABSTRACT: Dysregulation of professional APC has been postulated as a major mechanism underlying Ag-specific T cell hyporesponsiveness in patients with patent filarial infection. To address the nature of this dysregulation, dendritic cells (DC) and macrophages generated from elutriated monocytes were exposed to live microfilariae (mf), the parasite stage that circulates in blood and is responsible for most immune dysregulation in filarial infections. DC exposed to mf for 24–96 h showed a marked increase in cell death and caspase-positive cells compared with unexposed DC, while mf exposure did not induce apoptosis in macrophages. Interestingly, 48 h exposure of DC to mf induced mRNA expression of the pro-apoptotic gene TRAIL and both mRNA and protein expression of TNF-alpha. mAb to TRAIL-R2, TNF-R1

ORGANISM(S): Homo sapiens

SUBMITTER: Damien Chaussabel 

PROVIDER: E-GEOD-12787 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress