Transcription profiling of mouse cornea overexpressing PITX2A vs. wild type eyes
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ABSTRACT: Mice over-expressing human PITX2A in cornea by the keratocan upstream; regulatory sequences. Normalization procedure:; Data normalized to spikes. Values below 0.01 were set to 0.01. All of the genes in each sample were divided by the median of a user specified list of positive control genes (chip-to-chip normalization).
Project description:Mice over-expressing human PITX2A in cornea by the keratocan upstream regulatory sequences. Normalization procedure: Data normalized to spikes. Values below 0.01 were set to 0.01. All of the genes in each sample were divided by the median of a user specified list of positive control genes (chip-to-chip normalization). Keywords: repeat sample
Project description:The cornea is a protective and refractive barrier in the eye crucial for vision. Understanding the human cornea in health, disease and cell-based treatments can be greatly advanced with cornea organoids developed in culture from induced pluripotent stem cells. While a limited number of studies have investigated the single-cell transcriptomic composition of the human cornea, its organoids have not been examined similarly. Here we elucidated the transcriptomic cell fate map of 4-month-old human cornea organoids and human donor corneas. The organoids harbor cell clusters that resemble cells of the corneal epithelium, stroma and endothelium with sub-populations that capture signatures of early developmental states. Unlike the adult cornea where the largest cell population is stromal, the organoids contain large proportions of epithelial and endothelial-like cells. These corneal organoids offer a three-dimensional model to study corneal diseases and integrated responses of different cell types.