Identification of direct T-box target genes in the developing zebrafish mesoderm

ABSTRACT: The zebrafish genes spadetail (spt) and no tail (ntl) encode T-box transcription factors that are important for early mesoderm development. Although much has been done to characterize these genes, the identity and location of target regulatory elements remain largely unknown. Here, we survey the genome for downstream target genes of the Spt and Ntl T-box transcription factors. We find evidence for extensive additive interactions towards gene activation and limited evidence for combinatorial and antagonistic interactions between the two factors. Using in vitro binding selection assays to define Spt- and Ntl-binding motifs, we searched for target regulatory sequence via a combination of binding motif searches and comparative genomics. We identified regulatory elements for tbx6 and deltaD, an

ORGANISM(S): Danio rerio

SUBMITTER: Fiona Wardle 

PROVIDER: E-GEOD-12857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress