Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Control of Virulence Gene Expression in Citrobacter rodentium by Trascription Factor RegA

ABSTRACT: Identification of the targets of RegA with and without bicarbonate stimulation by comparing RegA knockout to multicopy RegA transgenics. RegA is an AraC like transcription factor identified in a mutational screen for virulence genes in Citrobacter rodentium, an attaching and effacing pathogen that causes transmissible colonic hyperplasia in mice. This experiment compares the RegA null strain with a multicopy plasmid rescue of this null strain in the presence and absence of bicarbonate with the aim of identifying pathogenesis related genes related to the early and late stages of attachment and effacement. Keywords: genetic modification, transcription factor, induction A strain of Citrobacter rodentium with a knockout of RegA was compared to the same strain rescued with a multicopy plasmid containing the wildtype RegA gene. These strains were analyzed with and without bicarbonate in an unconnected two factor design with dye balanced biological replicates.

ORGANISM(S): Citrobacter rodentium

SUBMITTER: Roy Robins-Browne 

PROVIDER: E-GEOD-12876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

RegA, an AraC-like protein, is a global transcriptional regulator that controls virulence gene expression in Citrobacter rodentium.

Hart Emily E   Yang Ji J   Tauschek Marija M   Kelly Michelle M   Wakefield Matthew J MJ   Frankel Gad G   Hartland Elizabeth L EL   Robins-Browne Roy M RM  

Infection and immunity 20080902 11

Citrobacter rodentium is an attaching and effacing pathogen which causes transmissible colonic hyperplasia in mice. Infection with C. rodentium serves as a model for infection of humans with enteropathogenic and enterohemorrhagic Escherichia coli. To identify novel colonization factors of C. rodentium, we screened a signature-tagged mutant library of C. rodentium in mice. One noncolonizing mutant had a single transposon insertion in an open reading frame (ORF) which we designated regA because of  ...[more]

Similar Datasets

Unknown Control of Virulence Gene Expression in Citrobacter rodentium by Trascription Factor RegA
2008-09-22 | GSE12876 | GEO
Transcriptomics Analysis of the evolutionary adaptation of an AraC-like global regulatory protein in the murine enteric pathogen Citrobacter rodentium
2015-02-19 | E-GEOD-57689 | biostudies-arrayexpress
Transcriptomics RNA-Seq of C. rodentium
2011-02-12 | E-MTAB-502 | biostudies-arrayexpress
Transcriptomics Gene expression in IL-10-deficient and wild-type mice following Citrobacter rodentium infection
2014-03-12 | E-GEOD-55812 | biostudies-arrayexpress
Transcriptomics Genome-wide Analysis of the Pho Regulon in a pstCA mutant of Citrobacter rodentium
2012-12-25 | E-GEOD-40906 | biostudies-arrayexpress
Transcriptomics The Nlrp12 inflammasome negatively regulates NOD2-mediated adjuvanticity, endotoxic shock and host defence afforded by an immunomodulatory muropeptide.
2015-07-25 | E-GEOD-59940 | biostudies-arrayexpress
Unknown Colonic gene expression in healthy and C. rodentium-infected mice lacking epithelial MHCII
2020-02-08 | GSE144952 | GEO
Unknown Mutation analysis of parental and 10 avirulent C. rodentium isolates from the feces of C3H/HeJ mice
2018-09-21 | GSE116595 | GEO
Unknown Transcriptome analysis of infected and uninfected mice with Citrobacter rodentium
2018-09-21 | GSE111097 | GEO
Unknown The Nlrp12 inflammasome negatively regulates NOD2-mediated adjuvanticity, endotoxic shock and host defence afforded by an immunomodulatory muropeptide.
2015-07-25 | GSE59940 | GEO