Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression following miR-30a knockdown in bipotential mouse embryonic liver (BMEL) cells

ABSTRACT: The goal was to identify genes targeted by miR-30a. Keywords: Transfection of cultured cells with antisense oligonucleotide RNA was isolated from BMELs tranfected with a locked nucleic acid (LNA) antisense oligonucleotide to miR-30a or with a scrambled control oligo.

ORGANISM(S): Mus musculus

SUBMITTER: Joshua Friedman

PROVIDER: E-GEOD-12908 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The microRNA-30 family is required for vertebrate hepatobiliary development.

Hand Nicholas J NJ Master Zankhana R ZR Eauclaire Steven F SF Weinblatt Daniel E DE Matthews Randolph P RP Friedman Joshua R JR

Gastroenterology 20081209 3

<h4>Background & aims</h4>The function of microRNA (miRNA) in liver development is unknown. To address this issue, we characterized miRNA expression in the embryonic mouse liver, performed functional miRNA analysis in zebrafish larvae, and identified novel hepatic miRNA targets.<h4>Methods</h4>Hepatic RNA isolated from mice at embryonic days 15.5, 18.5, and postnatal day 2 was hybridized to a mouse miRNA microarray. The microarray results were confirmed by Northern blot hybridization and quantit ...[more]

PMID: 19185580

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Project description:From the results of microarray, 7616 miRNAs with significantly different expression ratios were selected when CNE-2-S-18/SP cells were treated with DC120. Among these, 80 miRNAs were up-regulated and 78 were down-regulated. We then chose 20 miRNAs through the qRT M-bM-^@M-^S PCR, and our results showed that 9 miRNAs were up-regulated after treatment with DC120, which was consistent with the results of the microarray. Among these, miR-30a were significantly up-regulated. These results indicated that miR 30a expression was up-regulated by DC120. We then investigated the role of miR 30a in DC120-treated cells, we explored its potential targets using a bioinformatics approach of complementary base pairing. The relative luciferase activity of the reporter that contained the wild type 3' UTR was significantly suppressed when miR 30a was contransfected. In contrast, the luciferase activity of the mutant reporter was unaffected by the contransfection of miR 30a, indicating that miR 30a suppressed Sox2 gene expression using the miR 30a binding sequence at the 3' UTR of the Sox2 gene. Additionally, the ectopic expression of miR 30a caused a decrease in Sox2 protein expression and mRNA levels in CNE-2-S-18 cells. These data demonstrate that miR 30a may directly target the Sox2 gene via seed matches on both 3' UTR. Taken together, our results demonstrate that DC120 regulates Sox2 via up-regulation of miR 30a. In this study, two samples were used to acquire the miRNA expression profiling. We analyzed the differences in miRNA profiles between control (DMSO solution) and DC120 treatment in CNE-2-S-18/SP cells from miRNA microarrays. The CNE-2-S-18/SP cells were separately cultured with a DMSO solution or 10M-NM-<mol/L DC120 for 15h, and total RNA from all of the cells was extracted. Then we chose 20 up-regulated miRNAs through the qRT M-bM-^@M-^S PCR, and our results showed that 9 miRNAs were up-regulated after treatment with DC120. Among these, miR-30a were significantly up-regulated. Then we investigated the role of miR 30a in DC120-treated cells by bioinformatic methods and Dual luciferase reporter system, the function of miR-30a was analyzed through ectopic expression of miR 30a. Taken together, our results demonstrate that DC120 regulates Sox2 via up-regulation of miR 30a.

Dataset Information

Gene expression following miR-30a knockdown in bipotential mouse embryonic liver (BMEL) cells

Publications

The microRNA-30 family is required for vertebrate hepatobiliary development.

Similar Datasets

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