Profiling antibody function on antigen arrays

ABSTRACT: Humoral immune responses are traditionally characterized by determining the presence and quality of antibodies specific for certain antigens. Arraying of large numbers of antigens allows the parallel measurement of antibodies, generating patterns called antibody profiles. Functional characterization of these antibodies could help draw an even more informative map of an immune response. To generate functional antibody profiles we simultaneously tested not only IgM, IgG and IgA binding to but also complement activation by a panel of endogenous and exogenous antigens printed as microarrays, using normal and autoimmune human sera. We show that complement activation by a particular antigen in a given individual cannot be predicted by the measurement of antigen specific antibodies, in spite of a

ORGANISM(S): Homo sapiens

SUBMITTER: József Prechl 

PROVIDER: E-GEOD-12943 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress