Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genes upregulated by HLX

ABSTRACT: HLX was found as a VEGF-A induced gene in HUVEC (B.Schweighofer, submitted). In order to detect genes regulated by HLX HUVEC were infected by recombinant adenovirus expressing HLX for 4, 8, 16 and 32h. RNA was isolated and subjected to microarray analysis using Affymetrix microarray. HUVEC were infected with a MOI of 40 with either HLX expressing or empty adenovirus for 4, 8, 16 or 32 hours. HUVEC without an adenovirus infection were used as control.Total RNA was isolated with Trizol according to the instructions of the manufacturer. Gene expression was analysed with Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays according to the Affymetrix protocol.

ORGANISM(S): Homo sapiens

SUBMITTER: Julia Testori 

PROVIDER: E-GEOD-13054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The VEGF-regulated transcription factor HLX controls the expression of guidance cues and negatively regulates sprouting of endothelial cells.

Testori Julia J   Schweighofer Bernhard B   Helfrich Iris I   Sturtzel Caterina C   Lipnik Karoline K   Gesierich Sabine S   Nasarre Patrick P   Hofer-Warbinek Renate R   Bilban Martin M   Augustin Hellmut G HG   Hofer Erhard E  

Blood 20110111 9

The HLX gene encoding a diverged homeobox transcription factor has been found to be up-regulated by vascular endothelial growth factor-A (VEGF-A) in endothelial cells. We have now investigated the gene repertoire induced by HLX and its potential biologic function. HLX strongly increased the transcripts for several repulsive cell-guidance proteins including UNC5B, plexin-A1, and semaphorin-3G. In addition, genes for transcriptional repressors such as HES-1 were up-regulated. In line with these fi  ...[more]

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