Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

The effects of temporally restricted feeding on hepatic gene expression of Cry1, Cry2 double KO mice

ABSTRACT: Restricted feeding impacts the hepatic circadian clock of WT mice. Cry1, Cry2 double KO mice lack a circadian clock and are thus expected to show rhythmical gene expression in the liver. Imposing a temporally restricted feeding schedule on these mice shows how the hepatic circadian clock and rhythmic food intake regulate rhythmic transcription in parallel Cry1, Cry2 double KO mice were entrained either to ad libitum or temporally restricted feeding (tRF) schedules. Food was made available to mice under the tRF regimen only between ZT(CT)1 and ZT(CT)9. Mice were then released into constant darkness while the respective feeding schedules were still maintained. Liver tissue was collected on the second day of constant darkness at the indicated timepoints. Total RNA was extracted and 5ug of RNA was used in the standard Affymetrix protocol for amplification, labeling and hybridization

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Vollmers 

PROVIDER: E-GEOD-13062 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Time of feeding and the intrinsic circadian clock drive rhythms in hepatic gene expression.

Vollmers Christopher C   Gill Shubhroz S   DiTacchio Luciano L   Pulivarthy Sandhya R SR   Le Hiep D HD   Panda Satchidananda S  

Proceedings of the National Academy of Sciences of the United States of America 20091125 50

In mammals, the circadian oscillator generates approximately 24-h rhythms in feeding behavior, even under constant environmental conditions. Livers of mice held under constant darkness exhibit circadian rhythm in abundance in up to 15% of expressed transcripts. Therefore, oscillations in hepatic transcripts could be driven by rhythmic food intake or sustained by the hepatic circadian oscillator, or a combination of both. To address this question, we used distinct feeding and fasting paradigms on  ...[more]

Similar Datasets

Unknown The effects of temporally restricted feeding on hepatic gene expression of Cry1, Cry2 double KO mice
2009-11-23 | GSE13062 | GEO
Unknown Hepatic transcriptome by Next Generation Sequencing of WT and clock mutant mice fed a HFD ad libitum or time-restricted feeding.
2018-08-30 | GSE102072 | GEO
Unknown Genome-wide transcriptomic analysis of CRY2-KO and WT mouse articular cartilage
2019-06-19 | GSE132894 | GEO
Transcriptomics The effects of temporally restricted feeding on hepatic gene expression
2009-11-23 | E-GEOD-13060 | biostudies-arrayexpress
Proteomics The circadian E3 ligase complex SCFFBXL3+CRY targets TLK2
2019-11-12 | PXD009522 | Pride
Transcriptomics Expression data from primary mouse embryonic fibroblasts from wild-type and Cry double-knockout embryos
2011-09-30 | E-GEOD-31958 | biostudies-arrayexpress
Transcriptomics Feeding schedule and the circadian clock shape rhythms in hepatic gene expression
2009-11-12 | E-GEOD-13093 | biostudies-arrayexpress
Unknown Differential expression of genes affected by CRY1 or CRY2 in the absence of PER and NR1D proteins
2024-02-21 | GSE246853 | GEO
Unknown Expression data from primary mouse embryonic fibroblasts from wild-type and Cry double-knockout embryos
2011-10-01 | GSE31958 | GEO
Metabolomics,Multiomics Clock regulation of metabolites reveals coupling between transcription and metabolism
2017-04-05 | MTBLS292 | MetaboLights