Embryonic stem cell study

ABSTRACT: Cell culture: H9 hESCs were grown using feeder-free conditions for 7 days after passage then either RNA was harvested or embryoid bodies were prepared, cultured in suspension for 4 days, plated on gelatin-coated plates, and cultured for an additional 9 days in embryoid body medium. Detailed protocols for feeder-free culture and embryoid body formation are available at www.geron.com/scprotocols.pdf RNA preparation: Cells were then harvested in situ by overlaying with RLT lysis buffer (Qiagen, Valencia, CA). RNA was prepared using RNeasy Midi kits as described by the manufacture (Qiagen). To remove contaminants, RNA was precipitated by adding an equal volume of LiCl Precipitation Solution (Ambion, Austin, TX), washed with 70% ethanol and resuspended in water. Probe preparation: Probes were

ORGANISM(S): Homo sapiens

SUBMITTER: Chunhui Xu 

PROVIDER: E-GEOD-1310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress