Characterisation of Modular Reponse of non-pathogenic E.coli K12 MG1655 Response to acid adaptation, part B
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ABSTRACT: To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 2.5h at 0min, 10min, 30min, 1h, 1.5h, 2h, 2.5h
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and pH5.5 for 1hr every 5min.
Project description:To study the dynamics of acid adaptation in E.coli Keio collection mutant library at pH7 and pH5.5 after 15 minutes of adaptation Keywords: Single channel hybridisation were carried out using cy5 dye. Samples were collected from steady state system at pH7 and at pH5.5 after 15 minutes of adaptation
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course
Project description:To study the dynamics of acid adaptation in E.coli at pH5.5 as opposed to that at pH7, samples were collected from a steady-state system for transcriptomics. Keywords: time course
Project description:To study the dynamics of acid adaptation in E.coli Keio collection mutant library at pH7 and pH5.5 after 15 minutes of adaptation Keywords: Single channel hybridisation were carried out using cy5 dye.
Project description:S. enterica sv Kentucky 3795 and S. enterica sv Enteritidis NalR were grown to mid-log phase in TSB, then subjected to three different acidic conditions generated by two different acids: HCl to pH4.5, HCl to pH5.5 and CH3COOH to pH5.5. After 10min, total RNA was harvested und compared to total RNA harvested from identical control cultures grown in TSB without the pH alteration.
Project description:Enterohemorrhagic Escherichia coli (EHEC) is a gram negative enteric bacterial pathogen that can cause hemorrhagic colitis and heamolytic uremic syndrome (HUS) in humans and is the cause of bloody diarrhoea and acute renal failure in children. We have studied the transcriptional response of a colon cell line (CaCo2) to infection by EHEC and another closely related enteric pathogen Enteropathogenic Escherichia coli (EPEC) and compared its response to a cervical cell line (Hela). We carried out microarray analysis on CaCo2 infected with EHEC O157H:7 EDL933 and EPEC E2348/69 at 4 hours of infection and analysis on Hela infected with EHEC also at 4 hours of infection CaCo2 cells and Hela cells were grown to 80% confluency and infected with the bacteria for 4 hours before samples were collected for microarray analysis.