Transcription profiling of mouse embryonic stem cells (D3-pOCT-mESC) grown in low concentrations of nitric oxide

ABSTRACT: In order to identify the genes regulated in mouse embryonic stem cells by the effect of low concentrations of nitric oxide (NO), we analysed the transcriptome of cells treated with NO and compared it to those of cells cultured in the absence of leukemia inhibitory factor (LIF), and in the presence of LIF. We used the cell line D3-pOct4, which carries the enhanced Green Fluorescence Protein gene (eGFP) under the control of the Oct-4 promotor. This line is continuously maintained in the undifferentiated state in comparison with the wild type line in the presence of LIF. Experiment Overall Design: D3-pOct4 cells cultured in the presence of 1000 U/ml of LIF, in the absence of LIF, and in the absence of LIF with 2uM of diethylenetriamine nitric oxide adduct (DETA)/NO were grown for 7 days, main

ORGANISM(S): Mus musculus

SUBMITTER: Rafael Tapia Limonchi 

PROVIDER: E-GEOD-13235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress