SAGE Libraries from Highly Purified Mouse Hematopoietic Stem Cells from the E14.5 Fetal Liver and Adult Bone Marrow

ABSTRACT: Recent improvements in the development of methods for isolating functionally validated populations of nearly pure (>20%) murine hematopoietic stem cells (HSCs) have made it possible to analyze the molecular basis of the properties of these cells with increased precision. One intriguing feature of HSCs is the change they undergo in many of their key properties during development a change that affects the control of their self-renewal, cycling status, differentiated progeny output and steel factor sensitivity. To investigate how these differences are mediated, we undertook a genome-wide analysis of the transcripts present in highly purified fetal and adult HSCs using an adaptation of the LongSAGE methodology that allows its application to small numbers of cells (10 ng of RNA) by inclusion of

ORGANISM(S): Mus musculus

SUBMITTER: BCGSC BC Cancer Agency 

PROVIDER: E-GEOD-13243 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress