Comparative analysis of Drd1+ Medium Spiny Neurons and Drd2+ Medium Spiny Neurons

ABSTRACT: The cellular heterogeneity of the brain confounds efforts to elucidate the biological properties of distinct neuronal populations. Using Bacterial Artificial Chromosome (BAC) transgenic mice which express EGFP-tagged ribosomal protein L10a in defined cell populations, we have developed a methodology to affinity purify polysomal mRNAs from genetically defined cell populations in the brain. The utility of this approach is illustrated by the comparative analysis of four types of neurons, revealing hundreds of genes that distinguish these four cell populations. We find that even two morphologically indistinguishable, intermixed subclasses of medium spiny neuron display vastly different translational profiles and present examples of the physiological significance of such differences. This genet

ORGANISM(S): Mus musculus

SUBMITTER: Nathaniel Heintz 

PROVIDER: E-GEOD-13384 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress