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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human leukocytes or whole blood isolated from burn/trauma subjects using either buffy coat or PAXgene methods

ABSTRACT: #G017 Burn/Trauma Study. This is a Glue Grant Study comparison between gene expression analyses performed in eight trauma/burn subjects using either a buffy coat isolation of whole blood or using the PAXgene system

ORGANISM(S): Homo sapiens

SUBMITTER: Lyle Moldawer

PROVIDER: E-GEOD-1343 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Whole blood and leukocyte RNA isolation for gene expression analyses.

Feezor Robert J RJ Baker Henry V HV Mindrinos Michael M Hayden Doug D Tannahill Cynthia L CL Brownstein Bernard H BH Fay Adrian A MacMillan Sandra S Laramie Jason J Xiao Wenzhong W Moldawer Lyle L LL Cobb J Perren JP Laudanski Krzysztof K Miller-Graziano Carol L CL Maier Ronald V RV Schoenfeld David D Davis Ronald W RW Tompkins Ronald G RG

Physiological genomics 20041101 3

The analysis of gene expression data in clinical medicine has been plagued by the lack of a critical evaluation of accepted methodologies for the collection, processing, and labeling of RNA. In the present report, the reliability of two commonly used techniques to isolate RNA from whole blood or its leukocyte compartment was compared by examining their reproducibility, variance, and signal-to-noise ratios. Whole blood was obtained from healthy subjects and was either untreated or stimulated ex v ...[more]

PMID: 15548831

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Burn/Trauma Study

Project description:#G017 Burn/Trauma Study. This is a Glue Grant Study comparison between gene expression analyses performed in eight trauma/burn subjects using either a buffy coat isolation of whole blood or using the PAXgene system Keywords: other

2004-04-24 | GSE1343 | GEO

Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice

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Human buffy coat gene expression, custom 250-plex Nanostring panel

Project description:Buffy coat PBMC RNA expression was tested using a custom Nanostring probe panel, to identify general immune and glucocorticoid receptor-associated immune genes. ClinicalTrials.gov Identifier: NCT00824941

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Transcription profiling of mouse leukocytes from blood and spleen obtained from animals treated with trauma-hemorrhage, burn injury or lipopolysaccharide (LPS)-infusion

Project description:gene expression profiles of leukocytes from blood (WBCs) and spleen harvested at an early (two hours) time point after injury or sham injury in mice subjected to trauma-hemorrhage, burn injury or lipopolysaccharide (LPS)-infusion at three experimental sites

2008-06-12 | E-GEOD-2278 | biostudies-arrayexpress

Cord blood buffy coat DNA methylation is comparable to whole cord blood methylation

Project description:Cord blood DNA methylation is associated with numerous health outcomes and environmental exposures. Whole cord blood DNA reflects all nucleated blood cell types, while centrifuging whole blood separates red blood cells by generating a white blood cell buffy coat. Both sample types are used in DNA methylation studies. Cell types have unique methylation patterns and processing can impact cell distributions, which may influence comparability. To evaluate differences in cell composition and DNA methylation between buffy coat and whole cord blood samples, cord blood DNA methylation was measured with the Infinium EPIC BeadChip (Illumina) in 8 individuals, each contributing buffy coat and whole blood samples.

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Transcription profiling of mouse leukocytes from blood and spleen after burn injury or trauma hemorrhage

Project description:We report here the genes that are sequentially expressed in white blood cells from blood and spleen at 2 hours, 2 day,3 days, and 7 days after burn and sham injury or trauma-hemorrhage (T-H) and sham T-H. Experiment Overall Design: White blood cells (WBC) and splenocytes were harvested from individual mice and RNA was extracted, labeled and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChipsâ¢. The results were analyzed by dCHIP and BRB Array Tools software. Ingenuity Pathway Analysis (IPA) was applied to identify molecular networks significantly affected by the injuries.

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DNA methylation profiles of human buffy coat samples from Huntington's disease

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2020-07-02 | GSE146917 | GEO

Identification of plasma extracellular vesicle biomarkers after burn injury

Project description:Severe burn injury is a one of the most devastating forms of trauma with over 1.1 million burns each year requiring medical attention in the United States. Deaths from burn injury are commonly caused by immune-related sequelae such as pneumonia, organ failure and other opportunistic bacterial infections. Though there have been numerous studies to assess the immunological dysfunction associated with burn injury, there have yet to be a predictive biomarker, that can be used to assess high risk patients and their outcomes. We hypothesized that circulating extracellular vesicles (EVs) released early after burn injury would promote activate peripheral macrophages and specific cargo could be used as a biomarker to identify at-risk patients. To test this hypothesis, we assessed the immune consequences of adoptive transfer of EVs isolated after burn injury in vitro and used unbiased proteomic on EVs from mouse models and human burn patients from the UNC Jaycee Burn Center. Findings here suggest EVs serve as mediators of immune dysfunction and potential biomarkers.

2022-02-16 | PXD028515 | Pride

Identifying Non-Invasive Molecular Correlates relevant to Irradiation-Skin Burn Combined Injuries

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2015-05-15 | E-GEOD-66739 | biostudies-arrayexpress

Identifying Non-Invasive Molecular Correlates relevant to Irradiation-Skin Burn Combined Injuries

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