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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Influence of hyperthyroid conditions on gene expression in rat

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE13413: Influence of hyperthyroid conditions on gene expression in rat tibialis anterior GSE13414: Influence of hyperthyroid conditions on gene expression in rat extraocular muscles Refer to individual Series

ORGANISM(S): Rattus norvegicus

SUBMITTER: Thomas Postler

PROVIDER: E-GEOD-13436 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Influence of hyperthyroid conditions on gene expression in extraocular muscles of rats.

Postler Thomas S TS Budak Murat T MT Khurana Tejvir S TS Rubinstein Neal A NA

Physiological genomics 20090310 3

Extraocular muscles (EOMs) are a highly specialized type of tissue with a wide range of unique properties, including characteristic innervation, development, and structural proteins. Even though EOMs are frequently and prominently affected by thyroid-associated diseases, little is known about the direct effects of thyroid hormone on these muscles. To create a comprehensive profile of changes in gene expression levels in EOMs induced by thyroid hormone, hyperthyroid conditions were simulated by t ...[more]

PMID: 19276241

Similar Datasets

Influence of hyperthyroid conditions on gene expression in rat extraocular muscles

Project description:Extraocular muscles (EOMs) are a highly specialized type of tissue with a wide range of unique properties, including characteristic innervation, development, and structural proteins. Even though EOMs are frequently and prominently involved in thyroid-associated diseases, little is known about the immediate effects of thyroid hormone on these muscles. In order to create a comprehensive profile of changes in gene expression levels in EOMs induced by thyroid hormone, hyperthyroid conditions were simulated by treating adult Sprague-Dawley rats with intraperitoneal injections of 25 μg T3 per 100 g body weight over the course of six weeks; subsequently, microarray analysis was used to determine changes in mRNA levels in EOMs from T3-treated animals relative to untreated controls. Adult Sprague-Dawley rats (initial body weight 200 ± 40 g) were fed ad libitum with standard laboratory diet and tap water. Eight rats were randomly divided into a T3-treated and a control group. To simulate a chronically hyperthyroid state, T3 treatment was administered by intraperitoneal injections of 25 µg T3 (Sigma-Aldrich) per 100 g body weight. Injections were administered every second day over a period of six weeks. 24 hours after the last injection, the animals were sacrificed by CO2 asphyxiation. For RNA isolation, the extraocular muscles (EOMs) were removed, flash-frozen in liquid nitrogen and stored at -80 °C until further use.

2009-03-06 | E-GEOD-13414 | biostudies-arrayexpress

Influence of hyperthyroid conditions on gene expression in rat tibialis anterior

Project description:Extraocular muscles (EOMs) are a highly specialized type of tissue with a wide range of unique properties, including characteristic innervation, development, and structural proteins. Even though EOMs are frequently and prominently involved in thyroid-associated diseases, little is known about the immediate effects of thyroid hormone on these muscles. In order to create a comprehensive profile of changes in gene expression levels in EOMs induced by thyroid hormone, hyperthyroid conditions were simulated by treating adult Sprague-Dawley rats with intraperitoneal injections of 25 ?g T3 per 100 g body weight over the course of six weeks; subsequently, microarray analysis was used to determine changes in mRNA levels in EOMs from T3-treated animals relative to untreated controls. RNA isolated from tibialis anterior muscles of the same animals was used as a reference fast-twitch muscle. Adult Sprague-Dawley rats (initial body weight 200 ± 40 g) were fed ad libitum with standard laboratory diet and tap water. Eight rats were randomly divided into a T3-treated and a control group, three males and one female each. To simulate a chronically hyperthyroid state, T3 treatment was administered by intraperitoneal injections of 25 µg T3 (Sigma-Aldrich, St. Louis, MO, U.S.A.) per 100 g body weight. Injections were administered every second day over a period of six weeks. 24 hours after the last injection, the animals were sacrificed by CO2 asphyxiation, muscles were dissected immediately after death. Each of the four biological replicates in each group was used for a single microarray each. RNA from EOMs was used. Tibialis anterior RNA from the same animals served as a fast-twitch reference muscle.

2009-03-06 | E-GEOD-13413 | biostudies-arrayexpress

Rattus norvegicus

Project description:Influence of hyperthyroid conditions on gene expression in rat extraocular muscles

| PRJNA113985 | ENA

Project description:Influence of hyperthyroid conditions on gene expression in rat

| PRJNA110025 | ENA

Rattus norvegicus

Project description:Influence of hyperthyroid conditions on gene expression in rat tibialis anterior

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Laminar gene expression of rat extraocular orbital and global layers

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Extraocular muscle, expression changes in critical period (Porter lab)

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2003-07-16 | GSE497 | GEO

Influence of hyperthyroid conditions on gene expression in rat extraocular muscles

2009-03-06 | GSE13414 | GEO

Transcription profiling of microdissected global and orbital layers of the rectus extraocular muscle (EOM) from adult Sprague-Dawley rats

Project description:Adult rat global and orbital layers of rectal extraocular muscles microdissected using Arcturus Pixcell II LCM are gene profiled here using affymetrix rat RAE230A gene chip. www.med.upenn.edu/cellbio/faculty/rubinstein/; www.uphs.upenn.edu/pmi/members/khurana/lab%20page.htm

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Myasthenia gravis model: gene expression profile in diaphragm, extensor digitorum longus and extraocular muscles of rats

Project description:gene expression profile in diaphragm (DIA), extensor digitorum longus (EDL) and extraocular (EOM) muscles of rats with actively induced experimentally acquired MG (EAMG) using Affymetrix rat RAE230 gene chip. Keywords: myasthenia gravis (MG), rats with actively induced experimentally acquired MG (EAMG)

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