Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of pig jejunum from animals fed an animal or vegetable protein diet

ABSTRACT: This study examines the evidence that dietary can regulate genes in the intestinal epithelium and it discusses the physiologic relevance of such alterations in gene expression. Experiment Overall Design: Seven RNA samples were analyzed using Affymetrix GeneChipÂ® Porcine Genome Array (Affymetrix, Santa Clara, CA, USA) containing 23,937 probe sets that interrogate approximately 23,256 transcripts from 20,201 S. scrofa genes.

ORGANISM(S): Sus scrofa

SUBMITTER: yunzi sun

PROVIDER: E-GEOD-13457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:Transcriptomic analysis in response to Botrytis cinerea infections under conrasting nitrate regime Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants. To better understand the molecular changes underlying the impact of nitrate availability on plant susceptibility to B. cinerea, we performed plant transcriptome profiling assays on mock-treated (3 biological replicates) and infected plants ( 3 biological replicates) grown under three N conditions, using GeneChip Tomato Genome Arrays (Affymetrix).

Dataset Information

Transcription profiling of pig jejunum from animals fed an animal or vegetable protein diet

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets