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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human leukemia U937 cells response to hyperthermia

ABSTRACT: Hyperthermia (41ËC <) is widely used to treat patients with various cancers. Here, the effects of hyperthermia (42ËC for 90 min) on the gene expression in human lymphoma U937 cells were investigated using an Affymetrix GeneChip system. The cells were treated with hyperthermia (42Â°C for 90 min) and followed by incubation for 0, 1, 3 or 6 h at 37Â°C. The percentage of DNA fragmentation was 7.5 Â± 0.9 (mean Â± SD), 10.1 Â± 0.2, and 17.3 Â± 2.3 at the incubation periods of 1, 3, and 6 h, respectively. Of approximately 47,000 probe sets analyzed, the hyperthermia down-regulated 4,214 probe sets and up-regulated 1,334 by a factor 2.0 or greater. Experiment Overall Design: U937 cells, a human lymphoma cell line, were treated with hyperthermia (42Â°C for 90 min) and followed by incubation for 0, 1, 3 or 6 h at 37Â°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâs instructions.

ORGANISM(S): Homo sapiens

SUBMITTER: Yoshiaki Tabuchi

PROVIDER: E-GEOD-13503 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene profiling of the cell death induced by heat stress in HSC-3 human oral squamous carcinoma cells

Project description:Hyperthermia is widely used to treat patients with various cancers. The 42.5ËC is well known as inflection point of hyperthermia and generally up to 42ËC of hyperthermia is used in clinical case to combine with other therapy. Here, the effects of heat stress at 42 or 44ËC for 90 min on the gene expression in HSC-3 human oral squamous carcinoma cells were investigated using an Affymetrix GeneChip system. The cells were treated with heat stress (42 or 44Â°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37Â°C. The percentage of cell death was 5.0 Â± 1.5 (mean Â± SD) at 42Â°C for 12 h and 17.4 Â± 0.6 at 44Â°C for 12 h. Of approximately 47,000 probe sets analyzed, many genes that were differentially expressed by a factor 2.0 or greater were identified in the cells treated with heat stress at 42 and 44Â°C. HSC-3 human oral squamous carcinoma cells were treated with heat stress (42 or 44Â°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37Â°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChipÂ® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâs instructions.

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