Direct contact with mesenchymal stromal cells impacts CD133+ hematopoietic stem cells during ex-vivo expansion

ABSTRACT: Direct contact with mesenchymal stromal impacts on migratory behavior and gene expression profile of CD133+ hematopoietic stem cells during ex-vivo expansion Objective: To investigate the impact of direct contact between mesenchymal stromal cells (MSCs) and CD133+ hematopoietic stem cells (HSCs) in terms of expansion potential differentiation, migratory capacity and gene expression profile. Methods: CD133+ purified HSCs were cultured for 7 days on subconfluent MSCs supplemented with growth factor containing medium. After ex-vivo expansion, non-adherent and adherent cells were collected and analyzed separately. Results: The adherent cells were found to have a more immature phenotype compared to the non-adherent fraction. CXCR4 was up regulated in the adherent fraction which was associated

ORGANISM(S): Homo sapiens

SUBMITTER: Nael Alakel 

PROVIDER: E-GEOD-13566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress