Transcription profiling of mouse mesenchymal derived cells with miR-140 silenced or over-expressed to identify mir-140 targets

ABSTRACT: MicroRNAs (miRNAs) are short noncoding RNA molecules regulating the expression of mRNAs. Target identification of miRNAs is computationally difficult due to the relatively low homology between miRNAs and their targets. We present here an experimental approach to target identification where the cartilage-specific miR-140 was overexpressed and silenced in cells it is normally expressed in separate experiments. Expression of mRNAs was profiled in both experiments and the intersection of mRNAs repressed by miR-140 overexpression and derepressed by silencing of miR-140 was identified. The intersection contained only 49 genes, although both treatments affected the accumulation of hundreds of mRNAs. These 49 genes showed a very strong enrichment for the miR-140 seed sequence implying that the app

ORGANISM(S): Mus musculus

SUBMITTER: Helio Pais 

PROVIDER: E-GEOD-13590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress