Transcription profiling of Arabidopsis thaliana shoot apical meristem stem cell niche

ABSTRACT: A gene expression map of Arabidopsis thaliana shoot apical meristem stem cell niche was generated by isolating the specific cell type using the cell sorting methods. We used ap1-1;cal1-1 mutant background to enrich the sufficient number of cells for microarray analysis. Spatial and temporal regulation of gene expression is critical for stem-cell homeostasis. The shoot apical meristems of Arabidopsis thaliana harbor a small set of stem-cells which are surrounded by several million differentiating cells, imposing a severe limitation on the genomic analyses of stem-cell homeostasis. We have employed cell type-specific gene expression profiling that allowed us to generate a high-resolution gene expression map and it has revealed gene expression networks specific to the cell types of the stem-

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Venu Reddy 

PROVIDER: E-GEOD-13596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress