Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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AGO1-dependent Small RNA in Arabidopsis Identified by High-Throughput Sequencing

ABSTRACT: The goal of this study was to identify AGO1-dependent miRNA and tasiRNA in Arabidopsis by sequencing small RNA from wild type (Col-0) and ago1-25 plants. Keywords: Small RNA Sequencing-by-synthesis technology was used to sequence small RNA from flower tissue (stages 1-12) from wild-type (Col-0) and ago1-25 Arabidopsis thaliana plants. Three biological replicates were sequenced for both Col-0 and ago1-25.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: James Carrington 

PROVIDER: E-GEOD-13605 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

AGO1-miR173 complex initiates phased siRNA formation in plants.

Montgomery Taiowa A TA   Yoo Seong Jeon SJ   Fahlgren Noah N   Gilbert Sunny D SD   Howell Miya D MD   Sullivan Christopher M CM   Alexander Amanda A   Nguyen Goretti G   Allen Edwards E   Ahn Ji Hoon JH   Carrington James C JC  

Proceedings of the National Academy of Sciences of the United States of America 20081209 51

MicroRNA (miRNA)-guided cleavage initiates entry of primary transcripts into the transacting siRNA (tasiRNA) biogenesis pathway involving RNA-DEPENDENT RNA POLYMERASE6, DICER-LIKE4, and SUPPRESSOR OF GENE SILENCING3. Arabidopsis thaliana TAS1 and TAS2 families yield tasiRNA that form through miR173-guided initiation-cleavage of primary transcripts and target several transcripts encoding pentatricopeptide repeat proteins and proteins of unknown function. Here, the TAS1c locus was modified to prod  ...[more]

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