Expression Profiling to Identify Novel Desiccation Response Transcripts from Tortula ruralis gametophytes

ABSTRACT: In this study we have tried to utilize the unique aspects of the T. ruralis response to desiccation and rehydration to design a strategy to identify rehydrins that are of low abundance and perhaps completely novel to the desiccated or rehydration transcriptomes. We have constructed two Subtractive Suppression Hybridization (SSH) libraries (Diatchenko et al., 1996) that are designed to enrich for differentially expressed low-abundance transcripts contained within gametophytic cells either in the slow-dried state (mRNP sequestrated rehydrin transcripts) or cells that have been rapidly dried, rehydrated and sampled at 2h of hydration (rehydrin and recovery transcripts) when the translational change in gene expression is at its peak (Oliver 1991). To achieve this aim we constructed SSH librari

ORGANISM(S): Syntrichia ruralis

SUBMITTER: Scot Dowd 

PROVIDER: E-GEOD-13680 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress