Transcription profiling of E. coli planktonic samples exposed to cis-1,2-dichloroethylene (cis-DCE)

ABSTRACT: Metabolically-engineered Escherichia coli has been used previously to degrade the ubiquitous pollutant cis-1,2-dichloroethylene (cis-DCE), and the impact of the metabolic engineering was assessed by investigating the changes in the proteome. Here, genome-wide transcriptome analysis was performed to confirm that a strong heat shock and/or oxidative stress occurs during enhanced cis-DCE mineralization. Also, seven new stress proteins (YchH, YdeI, YodD, YodC, YgiW, YhcN, and YjaA) that were previously uncharacterized have been identified. Experiment Overall Design: Twelve planktonic RNA samples for the six sets of microarray experiments (set i was repeated) were prepared (Table 1 indicates the rationale of the whole-transcriptome studies): (i) cells of E. coli TG1 with TOM-Green/IsoILR1/GSHI

ORGANISM(S): Escherichia coli

SUBMITTER: Jintae Lee 

PROVIDER: E-GEOD-13698 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress