StaphVar-DNA microarray analysis of accessory genome of community-acquired methicillin resistant Staphylococcus aureus

ABSTRACT: Approximatively 75% of the genome of S. aureus (“core” genome) are highly conserved between strains, whereas the remaining 25% (“accessory” genome) are composed of variable regions that are mostly composed of mobile genetic elements (MGE), containing virulence and resistance genes. We have developed a composite DNA-microarray (StaphVar Array) which selectively targets 403 genes located on the accessory or core variable genome. Target genes encode antimicrobial resistance factors (35 %), virulence factors (28 %) and adhesins (31%). This microarray was validated with reference strains and used to characterize the genomic DNA of 13 community-acquired methicillin resistant S. aureus (CA-MRSA) strains representative of all the Multilocus Sequence Types (STs) described to date in Belgium. Analys

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Ricardo De Mendonca 

PROVIDER: E-GEOD-13702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress