Intracellular expression pattern of recombinant Mtb strain TB294 overexpressing sigma factor A

ABSTRACT: We previously showed that increased expression of the principal sigma factor, SigA, mediates the capacity of Mycobacterium tuberculosis 210 strains to grow more rapidly in human monocytes, compared to other strains. To identify SigA-regulated genes that favor intracellular mycobacterial growth, we used microarray analysis to compare gene expression between a recombinant M. tuberculosis 210 strain bearing a sense sigA construct and a vector control during growth in MonoMac6 cells. The most strongly upregulated gene in the sense sigA transformant was Rv2416c, which has previously been shown to enhance intracellular survival of M. smegmatis, and has been designated as eis. Real-time PCR confirmed marked upregulation of eis mRNA by the intracellular sense sigA transformant, and lysates of this

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Shiping Wu 

PROVIDER: E-GEOD-13780 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress