Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Time course effect of A-factor addition


ABSTRACT: Comparative hybridization of afsA deficient mutant versus afsA deficient mutant after A-factor addition 3 x dual channel slides, dye swap

ORGANISM(S): Streptomyces griseus subsp. griseus NBRC 13350

SUBMITTER: Sueharu Horinouchi 

PROVIDER: E-GEOD-13804 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus.

Hara Hirofumi H   Ohnishi Yasuo Y   Horinouchi Sueharu S  

Microbiology (Reading, England) 20090423 Pt 7


A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is a microbial hormone that triggers morphological differentiation and secondary metabolism in Streptomyces griseus. The effects of A-factor on global gene expression were determined by DNA microarray analysis of transcriptomes obtained with the A-factor-deficient mutant DeltaafsA. A-factor was added at a concentration of 25 ng ml(-1) to mutant DeltaafsA at the middle of the exponential growth phase, and RNA samples were prepared fro  ...[more]

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