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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression profiling of Ovine Herpesvirus 2-infected lymph nodes from Bos taurus

ABSTRACT: We hypothesized that the gene expression pattern of OvHV-2 may be important to understand the pathogenesis of malignant catarrhal fever (MCF). Therefore, RNA was extracted from lymph nodes of animals with MCF and healthy controls to be analyzed by a custom-made microarray. Two regions on the viral genome were transcriptionally active, one encoding a homologue to the latency-associated nuclear antigen (ORF73) of other gamma herpesviruses, the other with no predicted open reading frame. Keywords: Disease state analysis Two biological replicates with two technical replicates each, with one of the technical replicates being a dye-swap.

ORGANISM(S): Bos taurus

SUBMITTER: Claudia Meier-Trummer

PROVIDER: E-GEOD-13853 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Malignant catarrhal fever of cattle is associated with low abundance of IL-2 transcript and a predominantly latent profile of ovine herpesvirus 2 gene expression.

Meier-Trummer Claudia S CS Rehrauer Hubert H Franchini Marco M Patrignani Andrea A Wagner Ulrich U Ackermann Mathias M

PloS one 20090715 7

<h4>Background</h4>Malignant catarrhal fever (MCF) is a lethal disease of cattle, characterized by vasculitis, necrosis, and accumulation of activated, dysregulated cytotoxic lymphocytes in various tissues. Ovine gamma herpesvirus 2 (OvHV-2) is a causative agent of MCF, which may trigger the disease through immunopathogenic pathways. Lymphocytes are the main target of the virus. However, the pathogenic basis of the disease is still mysterious.<h4>Methods/findings</h4>We hypothesized that the gen ...[more]

PMID: 19603070

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Transcription profiling of Bos taurus lymph nodes upon infection with Ovine Herpesvirus 2

Project description:We hypothesized that the relative abundances of host cell transcripts in lymph nodes of animals with malignant catarrhal fever (MCF), compared to healthy controls, may be used to identify pathways that may help to explain the pathogenesis of MCF. Therefore, an abundance of host cell gene expression patterns in lymph nodes of animals with MCF and healthy controls were analyzed by microarray. Indeed, a vast number of genes related to inflammatory processes, lymphocyte activation, cell proliferation and apoptosis were detected at different abundances. However, the IL-2 transcript was eminent among the transcripts, which were, compared to healthy controls, less abundant in animals with MCF. Compared to healthy cattle, bovines with MCF appear to mimic an IL-2 knockout phenotype that has been described in mice. This supports the hypothesis that immunopathogenic events are linked to the pathogenesis of MCF. IL-2-deficiency may play an important role in the process. Experiment Overall Design: Comparison of lymph node samples from 3 infected animals with lymph node samples from 6 uninfected animals.

2009-08-09 | E-GEOD-13852 | biostudies-arrayexpress

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