Transcription profiling of rat ovarian granulosa cells - effect of the methoxychlor metabolite hpte in vitro

ABSTRACT: Ovarian granulosa cells play a central role in steroidogenesis, which is critical for female reproduction. Follicle-stimulating hormone (FSH) promotes cAMP–mediated signaling to regulate granulosa cell steroidogenesis. We have shown previously that 2, 2-bis-(p-hydroxyphenyl)-1, 1, 1-trichloroethane (HPTE) inhibits FSH- and dibutyryl cAMP-stimulated steroidogenesis, and affects the mRNA levels of steroidogenic pathway enzymes in rat granulosa cells. However, HPTE showed a differential effect in FSH- and cAMP-stimulated cells in that HPTE more completely blocked FSH- when compared to cAMP-driven steroidogenesis. The objective of this study was to analyze the effects of HPTE on global gene expression profiles in untreated granulosa cells and those challenged with FSH or cAMP. Granulosa cell

ORGANISM(S): Rattus norvegicus

SUBMITTER: Mehmet Uzumcu 

PROVIDER: E-GEOD-13883 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress