ChIP-chip analysis of NFI-1 in wildtype (N2) mixed stage C. elegans

ABSTRACT: The conserved Nuclear Factor I (NFI) family of transcription factors is unique to animals and essential for mammalian development. The C. elegans genome encodes a single NFI family member, whereas vertebrate genomes encode four distinct NFI protein subtypes (A, B, C, and X). NFI-1 deficient worms exhibit abnormalities including reduced lifespan, defects in movement and pharyngeal pumping, and delayed egg-laying. To explore the functional basis of these phenotypes, we sought to comprehensively identify NFI-1 binding targets in C. elegans. We first established NFI-1 DNA-binding specificity using an in vitro DNA-selection strategy. Analysis yielded a consensus motif of TTGGCA(N3)TGCCAA, which occurs 586 times in the genome, a 100-fold higher frequency than expected. We next asked which sites

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Christina Whittle 

PROVIDER: E-GEOD-13918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress