The contributions of sex, genotype and age to transcriptional variance in Drosophila melanogaster

ABSTRACT: Here we present a statistically rigorous approach to quantifying microarray expression data that allows the relative effects of multiple classes of treatment to be compared and incorporates analytical methods that are common to quantitative genetics. From the magnitude of gene effects and contributions of variance components, we find that gene expression in adult flies is affected most strongly by sex, less so by genotype and only weakly by age (for 1- and 6-wk flies); in addition, sex-genotype interactions may be present for as much as 10% of the Drosophila transcriptome. This interpretation is compromised to some extent by statistical issues relating to power and experimental design. Nevertheless, we show that changes in expression as small as 1.2 fold can be highly significant. Genotyp

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Stan Martin 

PROVIDER: E-GEOD-13928 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress