Expression of lignin-degrading enzymes in soils using targeted microarrays

ABSTRACT: Microarrays have become established tools for describing microbial systems, however the assessment of expression profiles for environmental microbial communities still presents unique challenges. Notably, the concentration of particular transcripts are likely very dilute relative to the pool of total RNA, and PCR-based amplification strategies are vulnerable to amplification biases and the appropriate primer selection. Thus, we apply a signal amplification approach, rather than template amplification, to analyze the expression of selected lignin-degrading enzymes in soil. Controls in the form of known amplicons and cDNA from Phanerochaete chrysosporium were included and mixed with the soil cDNA both before and after the signal amplification in order to assess the dynamic range of the mi

ORGANISM(S): Phanerochaete chrysosporium

SUBMITTER: Katrina Waters 

PROVIDER: E-GEOD-13977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress