Transcription profiling of mouse bFGF-treated JB6 cells and fully tumorigenic JB6 cells from the RT101 cell line - reversible and irreversible anchorage independent growth in skin cells

ABSTRACT: We have investigated the regulation of anchorage-independent growth (AIG) by basic fibroblast growth factor (bFGF) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) in JB6 mouse epidermal cells in the context of wound repair versus carcinogenesis responses. bFGF induces an unusually efficient but reversible AIG response, relative to TPA-induced AIG which is irreversible. Distinct global gene expression profiles are associated with anchorage-independent colonies arising from bFGF-stimulated JB6 cells, relative to colonies arising from fully tumorigenic JB6 cells (RT101), including genes exhibiting reciprocal regulation patterns. Thus, while TPA exposure results in commitment to an irreversible and tumorigenic AIG phenotype, the AIG response to bFGF is reversible with essentially complete r

ORGANISM(S): Mus musculus

SUBMITTER: Katrina Waters 

PROVIDER: E-GEOD-13979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress