Transcription profiling of mouse NMuMG cells after MTB/TRAS short-term induction, tumor, and TGFbeta treatment

ABSTRACT: To test whether SVD regression is sufficiently sensitive to detect activation of a secondary, endogenous pathway as it occurs following ectopic manipulation of a strong primary pathway by focusing on the relationship between the Ras and TGFβ signaling pathways. Experiment Overall Design: Bitransgenic MTB/TRAS mice in an FVB/N background were generated by crossing MTB and TRAS mice. To induce oncogenic v-H-Ras expression, 6-week old MTB/TRAS female mice were administered 2 mg/ml doxycycline with 5% sucrose in their drinking water. Mammary tissue was harvested at different post-induction time points and snap frozen. To generate Ras-driven tumors, MTB/TRAS mice were administered 0.012 mg/ml doxycycline in their drinking water and monitored for tumor formation. Mice were sacrificed when t

ORGANISM(S): Mus musculus

SUBMITTER: Lewis Chodosh 

PROVIDER: E-GEOD-13986 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress