Modulation of gene expression in U251 glioblastoma cells by binding of mutant p53 to intronic and intergenic sequences

ABSTRACT: Missense point mutations in the TP53 gene are frequent genetic alterations in human tumor tissue and cell lines derived thereof. Mutant p53 (mutp53) proteins have lost sequence-specific DNA binding, but have retained the ability to interact in a structure-selective manner with non-B DNA and to act as regulators of transcription. To identify functional binding sites of mutp53, we established a small library of genomic sequences bound by p53R273H in U251 human glioblastoma cells using chromatin immunoprecipitation (ChIP). Mutp53 binding to isolated DNA fragments confirmed the specificity of the ChIP. The mutp53 bound DNA sequences are rich in repetitive DNA elements, which are dispersed over non-coding DNA regions. Stable down-regulation of mutp53 expression strongly suggested that mutp53 bi

ORGANISM(S): Homo sapiens

SUBMITTER: Genrich Tolstonog 

PROVIDER: E-GEOD-13991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress