Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Transcription profiling of membrane-bound and cytosolic polysomes isolated from human MCF-7 cells

ABSTRACT: RNA from MCF-7 cells was fractionated by sucrose density gradient centrifugation to separate RNA associated with membrane-bound polysomes from RNA associated with free polysomes. These two populations were hybridized in triplicate to U133A microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Carol Westbrook 

PROVIDER: E-GEOD-1400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Membrane-associated and secreted genes in breast cancer.

Stitziel Nathan O NO   Mar Brenton G BG   Liang Jie J   Westbrook Carol A CA  

Cancer research 20041201 23

The identification of membrane-associated and secreted genes that are differentially expressed is a useful step in defining new targets for the diagnosis and treatment of cancer. Extracting information on the subcellular localization of genes represented on DNA microarrays is difficult and is limited by the incomplete sequence and annotation that is available in existing databases. Here we combine a biochemical and bioinformatic approach to identify membrane-associated and secreted genes express  ...[more]

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