Transcription profiling of mouse neurobastoma N18 cell line transfected with a pAd-Dyrk1a vector

ABSTRACT: The molecular mechanisms that lead to the cognitive defects characteristic of Down syndrome (DS), the most frequent cause of mental retardation, have remained elusive. Here we use a transgenic DS mouse model to show that DYRK1A gene dosage imbalance deregulates chromosomal clusters of genes located near neuron-restrictive silencer factor (REST/NRSF) binding sites. We found that DYRK1A binds the SWI/SNF-complex known to interact with REST/NRSF. Mutation of a REST/NRSF binding site in the promoter of the REST/NRSF target gene L1cam modifies the transcriptional effect of Dyrk1A-dosage imbalance on L1cam. DyrkA dosage imbalance perturbs Rest/Nrsf levels with decreased Rest/Nrsf expression in embryonic neurons and increased expression in adult neurons. We identified a coordinated deregulation o

ORGANISM(S): Mus musculus

SUBMITTER: Gilles Maussion 

PROVIDER: E-GEOD-14030 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress