Systematic identification of balanced transposition polymorphisms in Saccharomyces cerevisiae

ABSTRACT: High-throughput techniques for detecting DNA polymorphisms generally do not identify changes in which the genomic position of a sequence, but not its copy number, varies among individuals. To explore such balanced structural polymorphisms, we used array-based Comparative Genomic Hybridization (aCGH) to conduct a genome-wide screen for single-copy genomic segments that occupy different genomic positions in the standard laboratory strain of Saccharomyces cerevisiae (S90) and a polymorphic wild isolate (Y101) through analysis of six tetrads from a cross of these two strains. Paired-end high-throughput sequencing of Y101 validated four of the predicted rearrangements. The transposed segments contained one to four annotated genes each, yet crosses between S90 and Y101 yielded mostly viable tetr

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Caroline McCoach 

PROVIDER: E-GEOD-14223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress