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Genes induced by IL-9 in the colon of transgenic mice

ABSTRACT: The aim of this work was to identify genes induced by IL-9 in the colon of IL-9-tarnsgenic mice (Tg5). Therefore, we performed a comprehensive study of the genes expressed in the colon of IL-9 transgenic and wild type FVB mice, taking advantage of the affymetrix microarray technology. Keywords: cytokine-induced response RNA was extracted from a piece of the distal colon from IL-9 transgenic or wild type TVB mice. RNA samples from 4 different mice were pooled for each group before hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Jean-Christophe Renauld

PROVIDER: E-GEOD-14249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-9 promotes IL-13-dependent paneth cell hyperplasia and up-regulation of innate immunity mediators in intestinal mucosa.

Steenwinckel Valérie V Louahed Jamila J Lemaire Muriel M MM Sommereyns Caroline C Warnier Guy G McKenzie Andrew A Brombacher Frank F Van Snick Jacques J Renauld Jean-Christophe JC

Journal of immunology (Baltimore, Md. : 1950) 20090401 8

IL-9 contributes to lung inflammatory processes such as asthma, by promoting mast cell differentiation, B cell activation, eosinophilia, and mucus production by lung epithelial cells. The observation that IL-9 overexpressing mice show increased mast cell numbers in the intestinal mucosa suggests that this cytokine might also play a role in intestinal inflammation. In colons from IL-9 transgenic mice, the expression of Muc2, a major intestinal mucin gene, was up-regulated, together with that of C ...[more]

PMID: 19342650

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