Transcriptional profiling of equine chondrocyte de-differentiation in culture

ABSTRACT: Monolayer cultures of primary chondrocytes undergo morphological changes that have been broadly characterized as de-differentiation. Transcriptional profiling was used to evaluate changes in gene expression during this process. Articular cartilage was harvested from a single Standardbred horse; the chondrocytes were isolated and then maintained in monolayer culture for up to 14 days. Chondrocytes were collected on Day 1 and Day 14 of culture and snap frozen in liquid nitrogen. Total RNA was isolated, subjected to one round of linear RNA amplification, and then applied to an equine-specific cDNA microarray composed of 9322 elements. Three biological replicates were analyzed at each time point using a dye-swap experimental design. One hundred six transcripts were present at levels greater th

ORGANISM(S): Equus caballus

SUBMITTER: Michael Mienaltowski 

PROVIDER: E-GEOD-14255 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress