Gene expression profiling via LongSAGE in a non-model plant species: A case study in seeds of Brassica napus

ABSTRACT: Serial Analysis of Gene Expression (LongSAGE) was applied for gene expression profiling in seeds of oilseed rape (Brassica napus ssp. napus). The usefulness of this technique for detailed expression profiling in a non-model organism was demonstrated for the highly complex, neither fully sequenced nor annotated genome of B. napus by applying a tag-to-gene matching strategy based on Brassica ESTs and the annotated proteome of the closely related model crucifer A. thaliana. Transcripts from 3,094 genes were detected at two time-points of seed development, 23 days and 35 days after pollination (DAP). Differential expression in about 10 % of all matched genes, with a total abundance of 44 %, showed a shift from gene expression involved in diverse developmental processes including cell prolifera

ORGANISM(S): Brassica napus

SUBMITTER: Christian Obermeier 

PROVIDER: E-GEOD-14313 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress