Culture induced changes in Blood-Brain Barrier transcriptome: implications for amino acid transporters in vivo

ABSTRACT: Tight homeostatic control of brain amino acids (AA) depends on transport via solute family carrier proteins expressed by the Blood-Brain Barrier (BBB) microvascular endothelial cells (BMEC). To characterize the mouse BMEC transcriptome and probe culture-induced changes microarray analyses of PECAM-1+ endothelial cells (ppMBMECs) were compared with primary MBMECs (pMBMEC) cultured in the presence or absence of glial cells, and with b.End5 endothelioma cell-line. Selected cell marker and AA transporter mRNA levels were further verified by real-time RT PCR. Regardless of glial co-culture expression of a large subset of genes was strongly altered by a brief culture step. This is consistent with the known dependence of BMECs on in vivo interactions to maintain physiological functions, e.g. tigh

ORGANISM(S): Mus musculus

SUBMITTER: Francois Verrey 

PROVIDER: E-GEOD-14375 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress