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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human colorectal cancer cell lines transfected with homeobox gene CDX1 cDNA to identfiy downstream targets

ABSTRACT: This study is to identify downstream targets of homeobox gene CDX1. The study assayed the expression of 2 pairs of stably transfected colorectal cancer cell lines: The CDX1 nonexpressing CRC cell line HCT116 was stably transfected with either CDX1 cDNA in the pRC/CMV expression vector (HCT116-CDX1) or with vector control (HCT116-Vec). The CDX1-expressing CRC cell line LS174T was similarly transfected with either a pSilencer vector containing a short sequence of CDX1 siRNA (LS174T-siRNA) , or a pSilencer vector containing a scrambled siRNA sequence as a control (LS174T-Vec). Experiment Overall Design: 2 pairs of colorectal cancer cell lines were used for comparison

ORGANISM(S): Homo sapiens

SUBMITTER: Carol Chan

PROVIDER: E-GEOD-14380 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1

Project description:This study is to identify downstream targets of homeobox gene CDX1. The study assayed the expression of 2 pairs of stably transfected colorectal cancer cell lines: The CDX1 nonexpressing CRC cell line HCT116 was stably transfected with either CDX1 cDNA in the pRC/CMV expression vector (HCT116-CDX1) or with vector control (HCT116-Vec). The CDX1-expressing CRC cell line LS174T was similarly transfected with either a pSilencer vector containing a short sequence of CDX1 siRNA (LS174T-siRNA) , or a pSilencer vector containing a scrambled siRNA sequence as a control (LS174T-Vec).

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Homo sapiens

Project description:HCT116 (CRC): control siRNA vs. ZNF746 siRNA Transfected

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Expression data for human colon cancer cell line HCT116 transfected with siRNA targeting YAP1.

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HCT116 (CRC): control siRNA vs. ZNF746 siRNA Transfected

Project description:Transcriptional profiling of HCT116 cells compared to untreated control with HCT116 cells transfected with ZNF746 siRNA plasmid. Goal was to determine the effects of ZNF746 gene transfection on CRC progression. Two-condition experiment, HCT116 vs. ZNF746 siRNA.

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RNA-seq of FOXQ1-transfected HCT116 cells to understand the Wnt-dependent and independent FOXQ1 transcriptional output in colorectal cancers.

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