Transcriptomic and metabolomic profiles of the Plasmodium falciparum developmental cycle

ABSTRACT: In order to further our understanding of the metabolic network of the malaria parasite, Plasmodium falciparum, we carried out a concurrent transcriptomic and metabolomic study of the parasite's intraerythrocytic developmental cycle. These microarray data were generated to compare the expression levels of metabolic enzymes to the concentrations of their associated metabolites over the 48-hour life cycle. Sorbitol-synchronized Plasmodium falciparum (3D7 strain) was grown in tissue culture flasks in incubators according to standard protocols. Immediately after reinvasion, and at 8-hour intervals thereafter, parasites were harvested by centrifugation. For each timepoint, 0.5 mL of packed RBC (10% parasitemia) was pelleted by centrifugation, washed once in PBS and flash-frozen in liquid nitr

ORGANISM(S): Plasmodium falciparum

SUBMITTER: Joanne Morrisey 

PROVIDER: E-GEOD-14524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress