Transcription profiling of primary human hepatocytes - toxicogenomic comparison of TCDD and PCB 126 responsiveness in primary human hepatocytes

ABSTRACT: (Abstract) Toxicogenomics has great potential for enhancing our understanding of environmental chemical toxicity, hopefully leading to better-informed human health risk assessments. This study employed toxicogenomic technology to reveal species differences in response to two prototypical aryl hydrocarbon receptor (AHR) agonists, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the polychlorinated biphenyl (PCB) congener PCB 126. Dose responses of primary cultures of rat and human hepatocytes were determined using species-specific microarrays sharing over 4,000 gene orthologs. Forty-seven human and 79 rat genes satisfied dose response criteria for both chemicals and were subjected to further analysis including the calculation of EC50 and the relative potency (REP) of PCB 126 for each gene. On

ORGANISM(S): Homo sapiens

SUBMITTER: Erik Carlson 

PROVIDER: E-GEOD-14553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress