Transcription profiling of mouse normal thymus, thymus tumor, and XIST resistant thymus tumor

ABSTRACT: The non-coding Xist RNA triggers silencing of one of the two female X chromosomes during X inactivation in mammals. Gene silencing by Xist is restricted to special developmental contexts found in cells of the early embryo and specific hematopoietic precursors. The absence of critical silencing factors might explain why Xist cannot silence outside these contexts. Here, we show that Xist can also initiate silencing in a lymphoma model. Using the tumor context we identify the special AT rich binding protein SATB1 as an essential silencing factor. We show that loss of SATB1 in tumor cells abrogates the silencing function of Xist. In normal female lymphocytes Xist localizes along SATB1 filaments and, importantly, forced Xist expression can relocalize SATB1 into the Xist cluster. This reciprocal

ORGANISM(S): Mus musculus

SUBMITTER: christian haslinger 

PROVIDER: E-GEOD-14585 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress