Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bifidobacterium longum mutants and resistance to Heat-Shock

ABSTRACT: Transcriptional profiling of Bifidobacterium longum mutants versus wt strain in exponentional phase, with or without heat-shock treatment, and in stationary phase Keywords: Characterization of natural mutants Two B. longum mutants (NCC2912 and NCC2913) were analysed versus the wt strain NCC2705 in three conditions : exponential phase 37Â°, exponential phase with 7 min 50Â° heat shock, stationary phase. Two biologic replicates and 2 technical replicates

ORGANISM(S): Bifidobacterium longum

SUBMITTER: Bernard Berger

PROVIDER: E-GEOD-14628 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

HspR mutations are naturally selected in Bifidobacterium longum when successive heat shock treatments are applied.

Berger B B Moine D D Mansourian R R Arigoni F F

Journal of bacteriology 20100101 1

The development of molecular tools allowed light to be shed on several widespread genetic mechanisms aiming at limiting the effect of molecular damage on bacterial survival. For some bacterial taxa, there are limited tools in the genetic toolbox, which restricts the possibilities to investigate the molecular basis of their stress response. In that case, an alternative strategy is to study genetic variants of a strain under stress conditions. The comparative study of the genetic determinants resp ...[more]

PMID: 19880603

Similar Datasets

Bifidobacterium longum mutant

Project description:Transcriptional profiling of Bifidobacterium longum mutant versus wt strain in exponentional phase Keywords: Characterization of natural mutant One B. longum mutant (HPR2) was analysed versus the wt strain NCC2705 in: exponential phase 37Â°,pH 6.0, MRS, headspace flushing with CO2. Three biological replicates.

2010-06-05 | E-GEOD-16039 | biostudies-arrayexpress

Physiological stability of Bifidobacterium longum NCC2705 under continuous culture conditions

Project description:A continuous culture of Bifidobacterium longum NCC2705 was carried out in a 2.5-l reactor (Bioengineering AG, Wald, Switzerland), equipped with a Biospectra control system (Biospectra AG, Schlieren, Switzerland) and containing 2 l of MRS, added of 0.05% cysteine, inoculated with 2 % (v/v) preculture. The temperature was maintained at 37Â°C and the pH at 6.0 by addition of 5 M NaOH. The culture was stirred constantly at 250 rpm using two rushton type propellers. Anaerobic conditions were maintained by flushing the headspace of the reactor with CO2. After 8 h in batch mode the culture was run in continuous mode at a dilution rate of 0.1 h-1. Fresh medium was added using a peristaltic pump (Alitea, Bioengineering AG, Wald, Switzerland), and fermented broth harvested with a second peristaltic pump (Alitea, Bioengineering AG, Wald, Switzerland) set at a slightly higher flow rate. A stabilization period of 90 h (corresponding to nine reactor volume changes) was operated prior culture monitoring (t=0). Aliquots of 2 ml taken at t=31, 134 and 211 h were centrifuged (4,000 g, 1 min, room temperature) for transcriptomic analysis. Supernatants were discarded and cell pellets snap frozen in liquid nitrogen and stored at -80ÂºC until RNA-extraction. Keywords: Time course of Bifidobacterium longum in continuous culture Bifidobacterium longum NCC2705 at time 31 versus time 134 h and versus time 211 h in continuous culture. Two technical replicares with dyes swaps

2010-05-19 | E-GEOD-14896 | biostudies-arrayexpress

Lactobacillus johnsonii NCC comparative genomics with L. iatae

Project description:Whole genome DNA microarray designed for the probiotic L. johnsonii strain NCC533 was used for comparative genomic hybridization (CGH) of L. johnsonii ATCC 33200T, L. johnsonii BL261, L. gasseri ATCC 33323T and L. iatae BL263 (CECT 7394T). In these experiments, the fluorescence ratio distributions obtained with L. iatae and L. gasseri showed characteristic inter-species profiles. The percentage of conserved L. johnsonii NCC533 genes was about 83% in the L. johnsonii strains comparisons and decreased to 51% and 47% for L. iatae and L. gasseri, respectively. These results confirmed the separate status of L. iatae from L. johnsonii at the level of species, and also that it is closer to L. johnsonii than L. gasseri. L. johnsonii, L. gasseri, and L. iatae strains were hybridized versus L. johnsonii NCC533, some with replicates

2010-11-24 | E-GEOD-21627 | biostudies-arrayexpress

Bifidobacterium longum mutants and resistance to Heat-Shock

Project description:Transcriptional profiling of Bifidobacterium longum mutants versus wt strain in exponentional phase, with or without heat-shock treatment, and in stationary phase Keywords: Characterization of natural mutants

2009-12-21 | GSE14628 | GEO

S. pyogenes wild type vs. CvfA- mutant

Project description:The overall gene expression of a CvfA- mutant of Streptococcus pyogenes grown in C medium was compared to that of the wild type (HSC5spc). Stationary phase cells (8 hrs post-inoculation) were used because the gene expression of the CvfA- mutant was most different from that of the wild type at stationary phase, while the difference of gene expression between the CvfA- mutant and the wild type was negligible at exponential phase. Two biological replicates (two different RNA sets extracted from the CvfA- mutant and the wild type at different day were used): 399773,399887,399888 vs. 399889,399890,399891 Technical replicates (Microarrays were hybridized with the same RNA with the same dye): 399773 and 399887; 399889 and 399890 Dye swap (Microarrays were hybridized with the same RNA but Cy3 and Cy5 dyes were swapped): 399773, 399887 vs. 399888; 399889, 399890 vs. 399891

2010-06-25 | E-GEOD-15938 | biostudies-arrayexpress

Genomic hybridization of Methanobacteria to identify the diversity in strains on manure and olive mill waste.

Project description:Population dynamics of methanogenic genera was investigated in pilot anaerobic digesters. Cattle manure and two-phase olive mill wastes were codigested at a 3:1 ratio in two reactors operated at 37 ï¾°C and 55 ï¾°C. Other two reactors were run with either residue at 37 ï¾°C. Sludge DNA extracted from samples taken from all four reactors on days 4, 14 and 28 of digestion was used for hybridisation with the AnaeroChip, an oligonucleotide microarray targeting those groups of methanogenic archaea that are commonly found under mesophilic and thermophilic conditions (Franke-Whittle et al. 2009, in press, doi:10.1016/j.mimet.2009.09.017).

2010-11-10 | E-MEXP-2453 | biostudies-arrayexpress

Long-range chromosome interactions mediated by cohesin shape circadian gene expression [4C]

Project description:Mammalian circadian rhythm is established by the negative feedback loops consisting of a set of clock genes, which lead to the circadian expression of thousands of downstream genes. As genome-wide transcription is organized under the high-order chromosome structure, it is unclear how circadian gene expression is influenced by chromosome structure. In this study, we focus on the function of chromatin structure proteins cohesin as well as CTCF (CCCTC-binding factor) in circadian rhythm. We analyzed the interactome of a Bmal1-bound enhancer upstream of a clock gene, Nr1d1, by 4C-seq and observed that cohesin binding sites are enriched in the interactome. Integrating circadian transcriptome data and cistrome data, we found that cohesin-CTCF co-binding sites tend to insulate the phases of circadian oscillating genes while cohesin-non-CTCF sites facilitate the interaction between circadian enhancer and promoter. A coarse-grained model integrating the long-range effect of cohesin and CTCF markedly improved our mechanistic understanding of circadian gene expression. This model is subsequently supported by our RNA-seq data from cohesin knockout cells. Cohesin is required at least in part for driving the circadian gene expression by facilitating the enhancer-promoter looping. Taken together, our study provided a novel insight into the relationship between circadian transcriptome and the high-order chromosome structure. 4C-seq of a Bmal1 enhancer in mouse liver

2016-04-04 | E-GEOD-68830 | biostudies-arrayexpress

Transcription profiling of two rat cell lines using different blocking reagents and doses to optimize cDNA microarray procedures without using an external standard

Project description:We performed a dye-swap microarray experiment using a total of 24 Rat 15k cDNA duplicates microarrays. The study included RNA from samples with high expected differential gene expression termed high contrasts compared to self-self hybridization (rat cell lines AR42J and NRK52E). We than optimized a pipeline to maximize the number of genes found differentially expressed between the two different RNA samples while at the same time making sure that the methodology finds no differentially expressed genes in selfself experiments with the same cell lines. In this high-contrast vs. self-self method (HCSSM) significantly expressed genes is determined by estimating the false discovery rate (FDR) using a null distribution obtained from self-self experiment. The optimization criterion requires only 4 microarrays per evaluation, and the effects of blocking reagent dose, filtering, and background corrections methodologies were investigated. In our experiments a dose of 250 ng LNA dT blocker gave the highest number of differentially expressed genes. When the criterion was used to evaluate steps in the computational analysis of microarray data we found that no background correction and weight based filtering gave the largest number of differentially expressed genes. Background correction method, however, has a greater influence on the number of differentially expressed genes found than filtering.

2007-01-19 | E-TABM-202 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs rpoN mutant at OD 3.3

Project description:Comparison between Lp Paris wt and rpoN mutant in exponential phase (OD 3.3). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-175 | biostudies-arrayexpress

Comparison of the pre-passage wild-type strain H99W with two mouse-passaged strains

Project description:Changes in gene expression of the serotype A pre-passage wild-type strain H99W with two mouse-passaged strains were examined during log-phase growth at 37 ÂºC. Analysis included two replicates of each comparison with a Cy3-Cy5 dye swap

2016-08-11 | E-GEOD-59581 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data