Expression profile in internodes 1, 5 and 9 from high and low brix plants for sucrose content

ABSTRACT: Summary The expression profile of internodes from high brix plant was compared to internodes from low brix plants. Mature (In9), intermediate (In5) and immature internodes (In1) were collected from two different Progenies and immediately frozen in liquid nitrogen. Total RNA was extracted using Trizol. Progeny 1 was derived from two intra-specific polycrosses, one among Saccharum officinarum genotypes and the other combining Saccharum spontaneum genotypes. For each generation, 500 individuals were sampled for brix content and gene expression. The extreme segregants were selected for further analysis. The F3 hybrid individuals selected for molecular studies were planted in a field in single rows of 5 m using standard sugarcane cultivation practices. Brix readings and tissue samples were coll

ORGANISM(S): Saccharum officinarum

SUBMITTER: Milton Nishiyama Jr 

PROVIDER: E-GEOD-14730 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress