Nutrient limitation-induced regulation of Phanaerochaete chrysosporium genes

ABSTRACT: Transcript profiles of Phanerochaete chrysosporium grown on carbon-limited medium, nitrogen-limited medium and replete medium. Array design based on the DoE's Joint Genome Institute's v2.1 annotation. Goal is to define genes involved in lignin degradation. Keywords: gene expression under three different culture conditions From a data set of 10,004 unique alleles, each Roche NimbleGen (Madison, WI) array featured 12 unique 60mer probes per gene, all in triplicate. (Seven gene models composed mostly of repetitive DNA were represented by only 2 to 11 60mers.) Total RNA was purified from the five abovementioned media. In short, cultures were harvested by filtering through Miracloth (Calbiochem, EMD Biosciences, Gibbstown, NJ), squeeze dried and snap frozen in liquid nitrogen. Pellets were sto

ORGANISM(S): Phanerochaete chrysosporium

SUBMITTER: Dan Cullen 

PROVIDER: E-GEOD-14735 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress