Transcription profiling of Caco-2 and T84 human cell lines treated with flagellin, lymphotox beta or TNF alpha
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ABSTRACT: 14 day old Caco-2 and T84 cells were treated for 4 hours with flagellin, lymphotox beta or TNF alpha, respectively, and their expression profile compared to non-stimulated cells.
Project description:14 day old Caco-2 and T84 cells were treated with flagellin, lymphotox beta or TNF alpha, respectively, and their expression profile compared to non-stimulated cells. Keywords: other
Project description:T84 cells were stimulated with TNF, or flagellin, or wildtype Salmonella. RNA were extracted from treated cells at 1, 2, 3, 4, 5, and 6 hour time points, labelled, and hybridized the arrays. Keywords: Human
Project description:The series was designed to obtain gene expression patterns of TNF-alpha stimulated CaCo-2 cells. Keywords: TNF-alpha stimulation This series represents a group of 18 samples in 2 groups: 10 samples derived from not stimulated CaCo-2 cells, 8 samples from CaCo-2 cells stimulated with TNF-alpha in final concentration of 5 ng/ml for 60 min.
Project description:Analysis of gene expression in Caco-2 intestinal epithelial cells stimulated with flagellated or aflagellated S. enteritidis or recombinant flagellin. Results provide insight into how flagellin mediates innate immune responses in intestinal epithelial cells. Keywords: Gene expression analysis
Project description:Sequencing of 5'ends of RNA molecules from Caco-2 cells +/- TNFa stimulation CAGE library construction from RNA extracted from Caco-2 cells
Project description:We stimulated Caco-2 cells, with the TLR5 agonist flagellin (Fl), with the chemical ER stress inducer thapsigargin (TG), or a combination of both (TGFl). This strategy mimics a pro-inflammatory response in the presence of ER stress.